Journal: Frontiers in Oncology

Article Title: CD47 Enhances Cell Viability and Migration Ability but Inhibits Apoptosis in Endometrial Carcinoma Cells via the PI3K/Akt/mTOR Signaling Pathway

doi: 10.3389/fonc.2020.01525

Figure Lengend Snippet: CD47 up-regulation inhibited apoptosis in HEC-1A and Ishikawa cells. (A,B) Up-regulation of CD47 inhibited cell apoptosis in Ishikawa cell line. (C,D) CD47 overexpression inhibited cell apoptosis in HEC-1A cell line. * p < 0.05.

Article Snippet: The cell cycle of transfected HEC-1A and Ishikawa cells were analyzed using the Cell Cycle and Apoptosis Analysis Kit (Beyotime Biotechnology, Shanghai, China), according to the manufacturer's protocol.

Techniques: Over Expression

Journal: Bioscience Reports

Article Title: Oxidative and endoplasmic reticulum stresses are involved in palmitic acid-induced H9c2 cell apoptosis

doi: 10.1042/BSR20190225

Figure Lengend Snippet: Effect of PA on apoptosis-related protein expression in H9c2 cells ( A ) Caspase 3 activity in H9c2 cells after treatment with various doses of PA (100–800 µM) for 24 h. ( B ) Relative BAX expression in H9c2 cells after treatment with various doses of PA (100–800 µM) for 24 h. BAX expression was normalized to β-actin levels. Data are presented as the mean ± S.E.M. of three independent experiments, * P <0.05, ** P <0.01 versus the control group.

Article Snippet: A CCK8 Assay Kit, ROS Assay Kit, and Annexin V-PE/PI Apoptosis Analysis Kit were purchased from the Beyotime Institute of Biotechnology (Shanghai, China).

Techniques: Expressing, Activity Assay, Control

Journal: Bioscience Reports

Article Title: Oxidative and endoplasmic reticulum stresses are involved in palmitic acid-induced H9c2 cell apoptosis

doi: 10.1042/BSR20190225

Figure Lengend Snippet: Effect of NAC on PA-induced apoptosis and ER stress of H9c2 cells at 24 h ( A ) ROS levels in H9c2 cells after treatments; ( B ) NOX2 expression levels in H9c2 cells after treatments. ( C,D ) Apoptosis analysis by flow cytometry. d1: necrotic cells; d2: late apoptotic cells; d3: live cells; d4: early apoptotic cells. ( E ) Caspase 3 activity in H9c2 cells after treatments; ( F ) BAX expression levels in H9c2 cells after treatments. ( G ) GRP78 expression levels in H9c2 cells after treatments; ( H ) CHOP expression levels in H9c2 cells after treatments. Protein expression levels were normalized to β-actin levels. PA: 400 µM palmitic acid; NAC: 2 mM N-acetylcysteine. Data are presented as the mean ± S.E.M. of three independent experiments, * P <0.05, ** P <0.01 versus the control group and # P <0.05, ## P <0.01 represent PA+NAC treated group versus the PA-treated group.

Article Snippet: A CCK8 Assay Kit, ROS Assay Kit, and Annexin V-PE/PI Apoptosis Analysis Kit were purchased from the Beyotime Institute of Biotechnology (Shanghai, China).

Techniques: Expressing, Flow Cytometry, Activity Assay, Control

Journal: Bioscience Reports

Article Title: Oxidative and endoplasmic reticulum stresses are involved in palmitic acid-induced H9c2 cell apoptosis

doi: 10.1042/BSR20190225

Figure Lengend Snippet: Effect of 4-PBA on PA-induced apoptosis and oxidative stress of H9c2 cells at 24 h ( A ) GRP78 expression levels in H9c2 cells after treatments. ( B ) CHOP expression levels in H9c2 cells after treatments. ( C,D ) Apoptosis analysis by flow cytometry. d1: necrotic cells; d2: late apoptotic cells; d3: live cells; d4: early apoptotic cells. ( E ) Caspase 3 activity in H9c2 cells after treatments. ( F ) BAX expression levels in H9c2 cells after treatments. ( G ) ROS levels in H9c2 cells after treatments. ( H ) NOX2 expression levels in H9c2 cells after treatments. Protein expression levels were normalized to β-actin levels. PA: 400 µM palmitic acid; 4-PBA: 500 nM 4-PBA. Data are presented as the mean ± S.E.M. of three independent experiments, * P <0.05, ** P <0.01 versus the control group and & P <0.05, && P <0.01 represent PA+4-PBA treated group versus the PA-treated group.

Article Snippet: A CCK8 Assay Kit, ROS Assay Kit, and Annexin V-PE/PI Apoptosis Analysis Kit were purchased from the Beyotime Institute of Biotechnology (Shanghai, China).

Techniques: Expressing, Flow Cytometry, Activity Assay, Control

Journal: Viruses

Article Title: In Vitro Model Systems of Coxsackievirus B3-Induced Myocarditis: Comparison of Commonly Used Cell Lines and Characterization of CVB3-Infected iCell ® Cardiomyocytes

doi: 10.3390/v13091835

Figure Lengend Snippet: ( a ) Comparison of CVB3 load in different cell lines. CVB3 RNA copy numbers per ng RNA were obtained by qRT-PCR in CVB3-infected HeLa cells, HL-1 cells, H9c2 cells and iCell ® Cardiomyocytes, eight hours pi. ( b ) Quantification of CVB3 RNA copy number per ng RNA by qRT-PCR in CVB3-infected iCell ® Cardiomyocytes in the time course of infection.

Article Snippet: The analysis of the mode of cell death of CVB3-infected iCell ® Cardiomyocytes did not provide evidence that these cells undergo apoptosis or pyroptosis.

Techniques: Quantitative RT-PCR, Infection

Journal: Viruses

Article Title: In Vitro Model Systems of Coxsackievirus B3-Induced Myocarditis: Comparison of Commonly Used Cell Lines and Characterization of CVB3-Infected iCell ® Cardiomyocytes

doi: 10.3390/v13091835

Figure Lengend Snippet: ( a ) Course of CVB3 infection in different cell lines. Brightfield images of CVB3-infected HeLa cells, HL-1 cells, H9c2 cells and iCell ® Cardiomyocytes. Cells were infected with MOI 10 and observed for 24 h. ( b ) Western blot analysis of caspase-1 (left panel, positive control: lysate of human heart tissue of a patient with myocarditis) and caspase-3 cleavage (right panel, positive control: iCell Cardiomyocytes treated with staurosporine) in different CVB3-infected cell lines eight hours pi (MOI 10).

Article Snippet: The analysis of the mode of cell death of CVB3-infected iCell ® Cardiomyocytes did not provide evidence that these cells undergo apoptosis or pyroptosis.

Techniques: Infection, Western Blot, Positive Control

Journal: Viruses

Article Title: In Vitro Model Systems of Coxsackievirus B3-Induced Myocarditis: Comparison of Commonly Used Cell Lines and Characterization of CVB3-Infected iCell ® Cardiomyocytes

doi: 10.3390/v13091835

Figure Lengend Snippet: ( a ) Electron microscopy images of iCell ® Cardiomyocytes (A, non-infected, B–D infected) and murine heart tissue (E, non-infected; F, infected). iCell ® Cardiomyocytes were analyzed eight hours post CVB3 infection (MOI 10) and compared with murine heart tissue of CVB3-infected mice obtained during acute infection (eight days pi). Yellow arrows indicate destruction of myofibrils; yellow triangles indicate structural changes in mitochondria; yellow asterisks indicate the evolvement of vesicular structures; black arrows (C, D) indicate the destruction of the cellular membrane, which was observed in > 90% of 100 analyzed cells. D illustrates end-stage infection with final cellular lysis. ( b ) LIVE/DEAD staining of iCell ® Cardiomyocytes with an amine-reactive dye for analysis of cell membrane integrity. Cells were infected with MOI 10 and analyzed after eight hours. The percentage of positively stained cells is indicated in the left lower corner, respectively.

Article Snippet: The analysis of the mode of cell death of CVB3-infected iCell ® Cardiomyocytes did not provide evidence that these cells undergo apoptosis or pyroptosis.

Techniques: Electron Microscopy, Infection, Lysis, Staining

Journal: Viruses

Article Title: In Vitro Model Systems of Coxsackievirus B3-Induced Myocarditis: Comparison of Commonly Used Cell Lines and Characterization of CVB3-Infected iCell ® Cardiomyocytes

doi: 10.3390/v13091835

Figure Lengend Snippet: ( a ) Quantification of viral load in iCell ® Cardiomyocytes after inhibition of RIPK3 kinase activity (GSK’872) and inhibition of RIPK1 kinase activity (necrostatin). ( b ) Western blot analysis of RIPK3 expression in CVB3-infected iCell ® Cardiomyocytes eight hours pi (MOI 10) (left panel). Densitometric analysis is shown in the right panel. Data were normalized to GAPDH. ( c ) Western blot analysis of RPK1 expression in CVB3-infected iCell ® Cardiomyocytes eight hours pi (MOI 10).

Article Snippet: The analysis of the mode of cell death of CVB3-infected iCell ® Cardiomyocytes did not provide evidence that these cells undergo apoptosis or pyroptosis.

Techniques: Inhibition, Activity Assay, Western Blot, Expressing, Infection

Journal: Viruses

Article Title: In Vitro Model Systems of Coxsackievirus B3-Induced Myocarditis: Comparison of Commonly Used Cell Lines and Characterization of CVB3-Infected iCell ® Cardiomyocytes

doi: 10.3390/v13091835

Figure Lengend Snippet: ( a ) Western blot analysis of RIPK1 expression in iCell ® Cardiomyocytes transfected with either pIRES-EGFP-2A or pIRES-EGFP-3C (left panel). GFP expression is shown in the right panel. ( b ) Western blot analysis of RIPK1 expression in CVB3-infected iCell ® Cardiomyocytes eight hours pi (MOI 10) after pre-incubation with the protease inhibitors E64d or pepstatin A. iCell ® Cardiomyocytes were pre-incubated for one hour with the inhibitors before CVB3 infection. Densitometric analysis is shown in the right panel. Data were normalized to GAPDH.

Article Snippet: The analysis of the mode of cell death of CVB3-infected iCell ® Cardiomyocytes did not provide evidence that these cells undergo apoptosis or pyroptosis.

Techniques: Western Blot, Expressing, Transfection, Infection, Incubation

Journal: Viruses

Article Title: In Vitro Model Systems of Coxsackievirus B3-Induced Myocarditis: Comparison of Commonly Used Cell Lines and Characterization of CVB3-Infected iCell ® Cardiomyocytes

doi: 10.3390/v13091835

Figure Lengend Snippet: ( a ) Western blot analysis of RIPK1 expression in CVB3-infected iCell ® Cardiomyocytes eight hours pi (MOI 10) after pre-treatment with DMSO or Z-VAD-FMK (pan-caspase inhibitor) (left panel). Densitometric analysis is shown in the right panel. Data were normalized to GAPDH. ( b ) Quantification of viral load after DMSO treatment. iCell ® Cardiomyocytes were pre-incubated with DMSO at different concentrations for one hour before CVB3 infection.

Article Snippet: The analysis of the mode of cell death of CVB3-infected iCell ® Cardiomyocytes did not provide evidence that these cells undergo apoptosis or pyroptosis.

Techniques: Western Blot, Expressing, Infection, Incubation

Journal: Molecular Biotechnology

Article Title: miR-324-3p Suppresses Hepatic Stellate Cell Activation and Hepatic Fibrosis Via Regulating SMAD4 Signaling Pathway

doi: 10.1007/s12033-024-01078-w

Figure Lengend Snippet: MiR-324-3p suppresses the activation of transforming growth factor (TGF)-β1- induced LX-2 cells in vitro. A Hepatic stellate cells (HSCs) were transfected with miR-324-3p mimic, and expression of the miR-324-3p was determined by real-time-quantitative polymerase chain reaction (RT-qPCR). B RT-qPCR to analyze the miR-324-3p expression in HSCs transfected with the miR-324-3p inhibitor. C Cell counting kit-8 (CCK-8) to analyze the proliferation of transforming growth factor (TGF)-β1-induced LX-2 cells with indicated treatment. D Flow cytometry to detect the cycle and apoptosis of TGF-β1-induced LX-2 cells. E , G Western blot (WB) assay and RT-qPCR to evaluate α-smooth muscle actin (α-SMA) and Vimentin expression in transfected HSC cells. F , H The α-SMA, and Vimentin levels in transfected cells were analyzed by WB assay as well as RT-qPCR. * P < 0.05 and ** P < 0.01 vs . Control group; # P < 0.05 and ## P < 0.01 vs . NC + TGF-β1 group

Article Snippet: Then, the apoptosis was detected using the Annexin V-FITC/PI Apoptosis Analysis Kit (Bestbio, China) and analyzed using a CytoFLEX flow cytometer (Beckman Coulter, USA).

Techniques: Activation Assay, In Vitro, Transfection, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Cell Counting, CCK-8 Assay, Flow Cytometry, Western Blot, Control